Reporter

Part:BBa_K950004:Design

Designed by: Jakob Matthes   Group: iGEM12_Tuebingen   (2012-07-29)

firefly luciferase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 588
    Illegal XbaI site found at 48
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 588
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 588
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 588
    Illegal XbaI site found at 48
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 588
    Illegal XbaI site found at 48
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 808


Design Notes

Due to one XbaI and one EcoRI site, this part has to be cut with NheI and SpeI (NheI has XbaI/SpeI-compatible ends).

Primers were designed to replace the C-terminal SKL aminoacids with KL.

Source

The original source is the Firefly luciferase, see [http://www.ncbi.nlm.nih.gov/nuccore/AB644228.1].

The C-terminal modification is covered by [http://www.ncbi.nlm.nih.gov/pubmed/14558144 One-step measurement of firefly luciferase activity in yeast].

References

[http://onlinelibrary.wiley.com/doi/10.1002/yea.1024/abstract Leskinen, P., Virta, M. and Karp, M. (2003), One-step measurement of firefly luciferase activity in yeast. Yeast, 20: 1109–1113. doi: 10.1002/yea.1024]